验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue |
Positive IP detected in | mouse brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
20953-1-AP targets KCNJ1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, ELISA Application Description |
文献引用应用 | IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | KCNJ1 fusion protein Ag15100 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | potassium inwardly-rectifying channel, subfamily J, member 1 |
别名 | KCNJ1, KIR1.1, ROMK, ROMK1 |
计算分子量 | 391 aa, 45 kDa |
观测分子量 | 75-80 kDa |
GenBank蛋白编号 | BC074752 |
基因名称 | KCNJ1 |
Gene ID (NCBI) | 3758 |
RRID | AB_10732600 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P48048 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KCNJ1, also called ROMK, is an inward-rectifying K+ channel responsible for basal (non-flow stimulated) K+ secretion. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium. This channel is activated by internal ATP and can be blocked by external barium. The calculated molecule weight is 45kDa, 20953-1-AP can detect the band around 75-80 kDa, it has been considered to be a dimer of KCNJ1.(PMID: 17804670; 7929082; 15767585)
实验方案
Product Specific Protocols | |
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WB protocol for KCNJ1 antibody 20953-1-AP | Download protocol |
IP protocol for KCNJ1 antibody 20953-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Am J Physiol Renal Physiol (Pro)Renin Receptor Regulates Potassium Homeostasis through a Local Mechanism. |