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验证数据展示

  • WB analysis of mouse brain using 20953-1-AP

    WB analysis of mouse brain using 20953-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20953-1-AP (KCNJ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 20953-1-AP

    IP experiment of mouse brain using 20953-1-AP

    IP result of anti-KCNJ1 (IP:20953-1-AP, 4ug; Detection:20953-1-AP 1:300) with mouse brain tissue lysate 3600ug.

KCNJ1 Polyclonal antibody

KCNJ1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 20953-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA and More (1)

Synonyms

KCNJ1, KIR1.1, ROMK, ROMK1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

20953-1-AP targets KCNJ1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen KCNJ1 fusion protein Ag15100 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name potassium inwardly-rectifying channel, subfamily J, member 1
Synonyms KCNJ1, KIR1.1, ROMK, ROMK1
Calculated Molecular Weight 391 aa, 45 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC074752
Gene Symbol KCNJ1
Gene ID (NCBI) 3758
RRIDAB_10732600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48048
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KCNJ1, also called ROMK, is an inward-rectifying K+ channel responsible for basal (non-flow stimulated) K+ secretion. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium. This channel is activated by internal ATP and can be blocked by external barium. The calculated molecule weight is 45kDa, 20953-1-AP can detect the band around 75-80 kDa, it has been considered to be a dimer of KCNJ1.(PMID: 17804670; 7929082; 15767585)

实验方案

Product Specific Protocols
WB protocol for KCNJ1 antibody 20953-1-APDownload protocol
IP protocol for KCNJ1 antibody 20953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Am Soc Nephrol

Activation of mTORC1 in Collecting Ducts Causes Hyperkalemia.

Authors - Zhenguo Chen
View Article

Am J Physiol Renal Physiol

(Pro)Renin Receptor Regulates Potassium Homeostasis through a Local Mechanism.

Authors - Chuanming Xu
View Article