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验证数据展示

  • IF Staining of HeLa using 16985-1-AP

    IF Staining of HeLa using 16985-1-AP

    Immunofluorescent analysis of HeLa cells using 16985-1-AP (KCNJ3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • WB analysis of mouse brain using 16985-1-AP

    WB analysis of mouse brain using 16985-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16985-1-AP (KCNJ3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

KCNJ3 Polyclonal antibody

KCNJ3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16985-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

KIR3.1, KGA, GIRK-1, GIRK1, GIRK 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16985-1-AP targets KCNJ3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KCNJ3 fusion protein Ag10581 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium inwardly-rectifying channel, subfamily J, member 3
别名 KIR3.1, KGA, GIRK-1, GIRK1, GIRK 1
计算分子量 501 aa, 57 kDa
GenBank蛋白编号BC022495
基因名称 KCNJ3
Gene ID (NCBI) 3760
RRIDAB_2878336
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48549
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KCNJ3 antibody 16985-1-APDownload protocol
IF protocol for KCNJ3 antibody 16985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols