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验证数据展示

  • WB analysis of A431 using 23712-1-AP

    WB analysis of A431 using 23712-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 23712-1-AP (KCNK18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 23712-1-AP

    IF Staining of A375 using 23712-1-AP

    Immunofluorescent analysis of A375 cells using 23712-1-AP (KCNK18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

KCNK18 Polyclonal antibody

KCNK18 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23712-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

TWIK-related individual potassium channel, TRIK, TRESK2, TRESK 2, TRESK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, A375 cells, MCF-7 cells
Positive IF/ICC detected inA375 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23712-1-AP targets KCNK18 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 KCNK18 fusion protein Ag20514 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium channel, subfamily K, member 18
别名 TWIK-related individual potassium channel, TRIK, TRESK2, TRESK 2, TRESK
计算分子量 384 aa, 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC152979
基因名称 KCNK18
Gene ID (NCBI) 338567
RRIDAB_2879309
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z418
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KCNK18, also named as TRESK and TRIK, is an outward rectifying potassium channel which produces rapidly activating outward rectifier K+ currents. It may function as background potassium channel that sets the resting membrane potential. KCNK18 is inhibited by arachidonic acid and other naturally occurring unsaturated free fatty acids. KCNK18 channel activity is enhanced by volatile anesthetics, such as isoflurane. The gene of KCNK18 maps to chromosome 10q26.11, and encodes a 384-amino acid protein with a predicted unmodified molecular weight of 44 kDa. This antibody can be used to detect endogenous KCNK18, however, the reason for the additional band of 130-140 kDa is still unknown.

实验方案

Product Specific Protocols
WB protocol for KCNK18 antibody 23712-1-APDownload protocol
IF protocol for KCNK18 antibody 23712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols