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KCNK18 Polyclonal antibody

KCNK18 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23712-1-AP

Print datasheet

Synonyms

TWIK-related individual potassium channel, TRIK, TRESK2, TRESK 2, TRESK

验证数据展示

  • WB analysis of A431 using 23712-1-AP

    WB analysis of A431 using 23712-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 23712-1-AP (KCNK18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 23712-1-AP

    IF Staining of A375 using 23712-1-AP

    Immunofluorescent analysis of A375 cells using 23712-1-AP (KCNK18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, A375 cells, MCF-7 cells
Positive IF/ICC detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23712-1-AP targets KCNK18 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen KCNK18 fusion protein Ag20514 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name potassium channel, subfamily K, member 18
Synonyms TWIK-related individual potassium channel, TRIK, TRESK2, TRESK 2, TRESK
Calculated Molecular Weight 384 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC152979
Gene Symbol KCNK18
Gene ID (NCBI) 338567
RRIDAB_2879309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z418
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KCNK18, also named as TRESK and TRIK, is an outward rectifying potassium channel which produces rapidly activating outward rectifier K+ currents. It may function as background potassium channel that sets the resting membrane potential. KCNK18 is inhibited by arachidonic acid and other naturally occurring unsaturated free fatty acids. KCNK18 channel activity is enhanced by volatile anesthetics, such as isoflurane. The gene of KCNK18 maps to chromosome 10q26.11, and encodes a 384-amino acid protein with a predicted unmodified molecular weight of 44 kDa. This antibody can be used to detect endogenous KCNK18, however, the reason for the additional band of 130-140 kDa is still unknown.

实验方案

Product Specific Protocols
WB protocol for KCNK18 antibody 23712-1-APDownload protocol
IF protocol for KCNK18 antibody 23712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols