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KCNK4 Polyclonal antibody

KCNK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27113-1-AP

Print datasheet

Synonyms

K2p4.1, KCNK4, TRAAK, TRAAK1, Two pore K(+) channel KT4.1

验证数据展示

  • WB analysis using 27113-1-AP

    WB analysis using 27113-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27113-1-AP (KCNK4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 27113-1-AP

    IHC staining of human brain using 27113-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27113-1-AP (KCNK4 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 27113-1-AP

    IHC staining of human brain using 27113-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27113-1-AP (KCNK4 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse eye tissue, rat eye tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27113-1-AP targets KCNK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen KCNK4 fusion protein Ag25931 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name potassium channel, subfamily K, member 4
Synonyms K2p4.1, KCNK4, TRAAK, TRAAK1, Two pore K(+) channel KT4.1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC033577
Gene Symbol KCNK4
Gene ID (NCBI) 50801
RRIDAB_2880761
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYG8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KCNK4 antibody 27113-1-APDownload protocol
IHC protocol for KCNK4 antibody 27113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Funct Integr Genomics

LncRNA SLC25A21-AS1 increases the chemosensitivity and inhibits the progression of ovarian cancer by upregulating the expression of KCNK4

Authors - Ke Huang
View Article