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KCNMB4 Monoclonal antibody

KCNMB4 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

4H7G3

Cat No : 60122-1-Ig

Print datasheet

Synonyms

BK channel subunit beta 4, BKbeta4, Hbeta4, K(VCA)beta 4, KCNMB4, Maxi K channel subunit beta 4, Slo beta 4

验证数据展示

  • WB analysis of fetal human brain using 60122-1-Ig

    WB analysis of fetal human brain using 60122-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 60122-1-Ig

    IHC staining of human brain using 60122-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 60122-1-Ig

    IHC staining of human brain using 60122-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

60122-1-Ig targets KCNMB4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen KCNMB4 fusion protein Ag5618 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name potassium large conductance calcium-activated channel, subfamily M, beta member 4
Synonyms BK channel subunit beta 4, BKbeta4, Hbeta4, K(VCA)beta 4, KCNMB4, Maxi K channel subunit beta 4, Slo beta 4
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC042446
Gene Symbol KCNMB4
Gene ID (NCBI) 27345
RRIDAB_2280832
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDQ86W47
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KCNMB4 antibody 60122-1-IgDownload protocol
IHC protocol for KCNMB4 antibody 60122-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

Cytoprotective effects of the flavonoid quercetin by activating mitochondrial BKCa channels in endothelial cells.

Authors - Rafał Paweł Kampa
View Article
humanWB

Mitochondrion

Deficiency of the BKCa potassium channel displayed significant implications for the physiology of the human bronchial epithelium

Authors - Kamila Maliszewska-Olejniczak
View Article