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KCNS2 Polyclonal antibody

KCNS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13443-1-AP

Print datasheet

Synonyms

KCNS2, KIAA1144, KV9.2

验证数据展示

  • WB analysis of Y79 using 13443-1-AP

    WB analysis of Y79 using 13443-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13443-1-AP (KCNS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human eye using 13443-1-AP

    IHC staining of human eye using 13443-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 13443-1-AP (KCNS2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human eye using 13443-1-AP

    IHC staining of human eye using 13443-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 13443-1-AP (KCNS2 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 13443-1-AP

    IP experiment of HepG2 using 13443-1-AP

    IP result of anti-KCNS2 (IP:13443-1-AP, 4ug; Detection:13443-1-AP 1:300) with HepG2 cells lysate 3400ug.

经过测试的应用

Positive WB detected inY79 cells, HepG2 cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13443-1-AP targets KCNS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KCNS2 fusion protein Ag4271 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name potassium voltage-gated channel, delayed-rectifier, subfamily S, member 2
Synonyms KCNS2, KIAA1144, KV9.2
Calculated Molecular Weight 477 aa, 54 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC027932
Gene Symbol KCNS2
Gene ID (NCBI) 3788
RRIDAB_2249596
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULS6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KCNS2 antibody 13443-1-APDownload protocol
IHC protocol for KCNS2 antibody 13443-1-APDownload protocol
IP protocol for KCNS2 antibody 13443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols