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验证数据展示

  • WB analysis of Y79 using 13443-1-AP

    WB analysis of Y79 using 13443-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13443-1-AP (KCNS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human eye using 13443-1-AP

    IHC staining of human eye using 13443-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 13443-1-AP (KCNS2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human eye using 13443-1-AP

    IHC staining of human eye using 13443-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 13443-1-AP (KCNS2 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 13443-1-AP

    IP experiment of HepG2 using 13443-1-AP

    IP result of anti-KCNS2 (IP:13443-1-AP, 4ug; Detection:13443-1-AP 1:300) with HepG2 cells lysate 3400ug.

KCNS2 Polyclonal antibody

KCNS2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13443-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

KCNS2, KIAA1144, KV9.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells, HepG2 cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13443-1-AP targets KCNS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KCNS2 fusion protein Ag4271 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium voltage-gated channel, delayed-rectifier, subfamily S, member 2
别名 KCNS2, KIAA1144, KV9.2
计算分子量 477 aa, 54 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC027932
基因名称 KCNS2
Gene ID (NCBI) 3788
RRIDAB_2249596
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KCNS2 antibody 13443-1-APDownload protocol
IHC protocol for KCNS2 antibody 13443-1-APDownload protocol
IP protocol for KCNS2 antibody 13443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols