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- KD/KO Validated
KCTD10 Polyclonal antibody
KCTD10 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells, U2OS cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
产品信息
27279-1-AP targets KCTD10 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | KCTD10 fusion protein Ag25971 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | potassium channel tetramerisation domain containing 10 |
| Synonyms | ULR061, Potassium channel tetramerization domain-containing protein 10, MSTP028, hBACURD3, BTB/POZ domain-containing protein KCTD10 |
| Calculated Molecular Weight | 313 aa, 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC040062 |
| Gene Symbol | KCTD10 |
| Gene ID (NCBI) | 83892 |
| RRID | AB_2880827 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3F6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KCTD10 is a member of the polymerase delta-interacting protein 1 (PDIP1) gene family, which consists of 3 members, PDIP1, KCTD10 and TNFAIP1. KCTD10 is inducible by TNF-α, interacts with PCNA and the small subunit (p50) of DNA polymerase δ. Previous reports have shown that KCTD10 is essential for mammalian development. Further functional studies showed that KCTD10 interacts with proliferating cell nuclear antigen (PCNA) and plays an important role in DNA synthesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KCTD10 antibody 27279-1-AP | Download protocol |
| IHC protocol for KCTD10 antibody 27279-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination.
| ||
Front Cell Dev Biol Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB.
| ||
Genet Res (Camb) Inhibition of KCTD10 Affects Diabetic Retinopathy Progression by Reducing VEGF and Affecting Angiogenesis
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