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验证数据展示

  • IHC staining of human breast cancer using 23345-1-AP

    IHC staining of human breast cancer using 23345-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 23345-1-AP

    WB analysis of HeLa using 23345-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 23345-1-AP

    IF Staining of HepG2 using 23345-1-AP

    Immunofluorescent analysis of HepG2 cells using 23345-1-AP (KDELC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

KDELC2 Polyclonal antibody

KDELC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23345-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Protein O-xylosyltransferase POGLUT3, Protein O-glucosyltransferase 3, POGLUT3, KDEL motif-containing protein 2, EC:2.4.2.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue, SMMC-7721 cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23345-1-AP targets KDELC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 KDELC2 fusion protein Ag19929 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KDEL (Lys-Asp-Glu-Leu) containing 2
别名 Protein O-xylosyltransferase POGLUT3, Protein O-glucosyltransferase 3, POGLUT3, KDEL motif-containing protein 2, EC:2.4.2.-
计算分子量 507 aa, 59 kDa
观测分子量 62 kDa
GenBank蛋白编号BC036526
基因名称 KDELC2
Gene ID (NCBI) 143888
RRIDAB_2879260
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z4H8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KDELC2 antibody 23345-1-APDownload protocol
IHC protocol for KDELC2 antibody 23345-1-APDownload protocol
IF protocol for KDELC2 antibody 23345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols