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KDM1 Monoclonal antibody, PBS Only

KDM1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F10A12

Cat no : 67037-1-PBS

Print datasheet

Synonyms

AOF2, BHC110, KDM1, KDM1A, KIAA0601, LSD1, lysine (K) demethylase 1

验证数据展示

  • IHC staining of human colon cancer using 67037-1-Ig (same clone as 67037-1-PBS)

    IHC staining of human colon cancer using 67037-1-Ig (same clone as 67037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KDM1 antibody included
  • WB analysis using 67037-1-Ig (same clone as 67037-1-PBS)

    WB analysis using 67037-1-Ig (same clone as 67037-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67037-1-Ig (KDM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KDM1 Ready-To-Use IHC Kit. KDM1 primary antibody included.

产品信息

67037-1-PBS targets KDM1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen KDM1 fusion protein Ag14778 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lysine (K)-specific demethylase 1
Synonyms AOF2, BHC110, KDM1, KDM1A, KIAA0601, LSD1, lysine (K) demethylase 1
Calculated Molecular Weight 876 aa, 95 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC040194
Gene Symbol KDM1
Gene ID (NCBI) 23028
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60341
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lysine specific demethylase 1 (LSD1/BHC110/KIAA0601/p110b/AOF2/KDM1) is an amine oxidase that catalyzes histone demethylation via a flavin adenine dinucleotide (FAD)-dependent oxidative reaction(PMID:19703393). This protein belongs to the flavin monoamine oxidase family. Its structure and function is conserved from yeast to human and it is typically associated to CoREST, a corepressor protein, and histone deacetylases HDAC1 and HDAC2. Human LSD1 consists of 852 amino acids and comprises an N-terminal SWIRM domain, involved in protein interactions, and a C-terminal amine oxidase domain, which contains an insertion that forms the CoREST interacting site (the so-called tower domain )(PMID:20164337). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 93-95 kDa and apparent molecular mass of 110 kDa.