验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24311-1-AP targets KDM2A in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | KDM2A fusion protein Ag19448 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | lysine (K)-specific demethylase 2A |
| Synonyms | CXXC8, F box protein FBL7, F box protein Lilina, F box/LRR repeat protein 11, FBL11, FBL7, FBXL11, JHDM1A, KDM2A, KIAA1004, LILINA, lysine (K) demethylase 2A, Lysine specific demethylase 2A |
| Calculated Molecular Weight | 1162 aa, 133 kDa |
| Observed Molecular Weight | 126 kDa |
| GenBank Accession Number | BC064360 |
| Gene Symbol | KDM2A |
| Gene ID (NCBI) | 22992 |
| RRID | AB_2879488 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2K7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KDM2A(Lysine-specific demethylase 2A) is also named as CXXC8, FBL7, FBXL11, JHDM1A, KIAA1004 and belongs to the JHDM1 histone demethylase family. It is required to maintain the heterochromatic state, and playing a role in epigenetic silencing. KDM2A is one of the four subunits of the E3 ubiquitin protein ligase complex (SCF) with SKP1, CUL1, RBX1, Fbox protein bringing ubiquitin conjugating enzymes to substrates recruited by F-box.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KDM2A antibody 24311-1-AP | Download protocol |
| IP protocol for KDM2A antibody 24311-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Oncol (Dordr) Hepatitis B virus core protein stabilizes RANGAP1 to upregulate KDM2A and facilitate hepatocarcinogenesis | ||
J Cell Mol Med Circular RNA circFOXO3 regulates KDM2A by targeting miR-214 to promote tumor growth and metastasis in oral squamous cell carcinoma. | ||
Cell Rep Enhancer RNAs Mediate Estrogen-Induced Decommissioning of Selective Enhancers by Recruiting ERα and Its Cofactor. | ||
Cell Death Dis CircRNF144B/miR-342-3p/FBXL11 axis reduced autophagy and promoted the progression of ovarian cancer by increasing the ubiquitination of Beclin-1 |
