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KDM3B Polyclonal antibody

KDM3B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 19915-1-AP

Print datasheet

Synonyms

JmjC domain-containing histone demethylation protein 2B, JHDM2B, EC:1.14.11.65, C5orf7, 5qNCA

验证数据展示

  • WB analysis using 19915-1-AP

    WB analysis using 19915-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19915-1-AP (KDM3B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 19915-1-AP

    IF Staining of A431 using 19915-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using KDM3B antibody (19915-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, human placenta tissue, HeLa cells, HepG2 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19915-1-AP targets KDM3B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen KDM3B fusion protein Ag13795 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysine (K)-specific demethylase 3B
Synonyms JmjC domain-containing histone demethylation protein 2B, JHDM2B, EC:1.14.11.65, C5orf7, 5qNCA
Calculated Molecular Weight 1761 aa, 192 kDa
Observed Molecular Weight 192 kDa, 85 kDa
GenBank Accession NumberBC001202
Gene Symbol KDM3B
Gene ID (NCBI) 51780
RRIDAB_10693529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7LBC6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KDM3B antibody 19915-1-APDownload protocol
IF protocol for KDM3B antibody 19915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Genet

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Authors - Yuan Li
View Article