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验证数据展示

  • WB analysis of NIH/3T3 using 24943-1-AP

    WB analysis of NIH/3T3 using 24943-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24943-1-AP (KDM4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 24943-1-AP

    WB analysis of MCF-7 using 24943-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24943-1-AP (KDM4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

KDM4A Polyclonal antibody

KDM4A Polyclonal Antibody for WB, ELISA
Cat No. 24943-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA and More (1)

别名

JHDM3A, JMJD2, JMJD2A, KDM4A, KIAA0677, lysine (K) demethylase 4A, Lysine specific demethylase 4A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24943-1-AP targets KDM4A in WB, chIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, chIP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 KDM4A fusion protein Ag20747 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lysine (K)-specific demethylase 4A
别名 JHDM3A, JMJD2, JMJD2A, KDM4A, KIAA0677, lysine (K) demethylase 4A, Lysine specific demethylase 4A
计算分子量 1064 aa, 121 kDa
观测分子量 55 kDa
GenBank蛋白编号BC002558
基因名称 KDM4A
Gene ID (NCBI) 9682
RRIDAB_2879812
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75164
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KDM4A also named as JHDM3A, JMJD2, or KIAA0677 is a 1066 amino acid protein, which contains one JmjC domain, two Tudor domains and two PHD-type zinc fingers. KDM4A localizes in nucleus and belongs to the JHDM3 histone demethylase family. KDM4A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. KDM4A also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. KDM4A exists as two isoforms. The short isoform of KDM4A in which the N-terminal demethylase domains has been deleted. The molecular weight of short isoform is 55Da.

实验方案

Product Specific Protocols
WB protocol for KDM4A antibody 24943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Folic Acid Attenuates Glial Activation in Neonatal Mice and Improves Adult Mood Disorders Through Epigenetic Regulation.

Authors - Tiantian Zhao
View Article
humanWB,chIP

Biochem Biophys Res Commun

The histone demethylase Kdm4 suppresses activation of hepatic stellate cell by inducing MiR-29 transcription.

Authors - Ming Kong
  • KD Validated
View Article