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  • Featured Product
  • KD/KO Validated

KDM4A Polyclonal antibody

KDM4A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 24943-1-AP

Print datasheet

Synonyms

JHDM3A, JMJD2, JMJD2A, KDM4A, KIAA0677, lysine (K) demethylase 4A, Lysine specific demethylase 4A

验证数据展示

  • WB analysis of NIH/3T3 using 24943-1-AP

    WB analysis of NIH/3T3 using 24943-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24943-1-AP (KDM4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 24943-1-AP

    WB analysis of MCF-7 using 24943-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24943-1-AP (KDM4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNIH/3T3 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24943-1-AP targets KDM4A in WB, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, chIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen KDM4A fusion protein Ag20747 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysine (K)-specific demethylase 4A
Synonyms JHDM3A, JMJD2, JMJD2A, KDM4A, KIAA0677, lysine (K) demethylase 4A, Lysine specific demethylase 4A
Calculated Molecular Weight 1064 aa, 121 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC002558
Gene Symbol KDM4A
Gene ID (NCBI) 9682
RRIDAB_2879812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75164
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KDM4A also named as JHDM3A, JMJD2, or KIAA0677 is a 1066 amino acid protein, which contains one JmjC domain, two Tudor domains and two PHD-type zinc fingers. KDM4A localizes in nucleus and belongs to the JHDM3 histone demethylase family. KDM4A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. KDM4A also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. KDM4A exists as two isoforms. The short isoform of KDM4A in which the N-terminal demethylase domains has been deleted. The molecular weight of short isoform is 55Da.

实验方案

Product Specific Protocols
WB protocol for KDM4A antibody 24943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Folic Acid Attenuates Glial Activation in Neonatal Mice and Improves Adult Mood Disorders Through Epigenetic Regulation.

Authors - Tiantian Zhao
View Article
humanWB,chIP

Biochem Biophys Res Commun

The histone demethylase Kdm4 suppresses activation of hepatic stellate cell by inducing MiR-29 transcription.

Authors - Ming Kong
  • KD Validated
View Article