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验证数据展示

  • WB analysis using 29977-1-AP

    WB analysis using 29977-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29977-1-AP (KDM4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 29977-1-AP

    FC experiment of A549 using 29977-1-AP

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KDM4A (29977-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IP experiment of MCF-7 using 29977-1-AP

    IP experiment of MCF-7 using 29977-1-AP

    IP result of anti-KDM4A (IP:29977-1-AP, 4ug; Detection:29977-1-AP 1:1500) with MCF-7 cells lysate 2120 ug.

KDM4A Polyclonal antibody

KDM4A Polyclonal Antibody for WB, FC (Intra), IP, ELISA
Cat No. 29977-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), IP, ELISA

别名

JMJD2, JmjC domain-containing histone demethylation protein 3A, JHDM3A, EC:1.14.11.66, [histone H3]-trimethyl-L-lysine(9) demethylase 4A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, mouse lung tissue, MCF-7 cells, MDA-MB-231 cells, NCl-H1299 cells
Positive IP detected inMCF-7 cells, A549 cells
Positive FC (Intra) detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29977-1-AP targets KDM4A in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 KDM4A fusion protein Ag31992 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lysine (K)-specific demethylase 4A
别名 JMJD2, JmjC domain-containing histone demethylation protein 3A, JHDM3A, EC:1.14.11.66, [histone H3]-trimethyl-L-lysine(9) demethylase 4A
计算分子量 1064 aa, 121 kDa
观测分子量130-150 kDa
GenBank蛋白编号BC002558
基因名称 KDM4A
Gene ID (NCBI) 9682
RRIDAB_2923625
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75164
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KDM4A also named as JHDM3A, JMJD2, or KIAA0677 is a 1066 amino acid protein, which contains one JmjC domain, two Tudor domains and two PHD-type zinc fingers. KDM4A localizes in nucleus and belongs to the JHDM3 histone demethylase family. KDM4A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. KDM4A also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. KDM4A exists as two isoforms. The short isoform of KDM4A in which the N-terminal demethylase domains has been deleted. The molecular weight of short isoform is 55Da.

实验方案

Product Specific Protocols
WB protocol for KDM4A antibody 29977-1-APDownload protocol
IP protocol for KDM4A antibody 29977-1-APDownload protocol
FC protocol for KDM4A antibody 29977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols