验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293T cells |
Positive IP detected in | HeLa cells |
Positive IF-P detected in | mouse small intestine tissue |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IF | See 2 publications below |
产品信息
18825-1-AP targets RBP2 in WB, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, IF-P, IP, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | lysine (K)-specific demethylase 5A |
别名 | KDM5A, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, EC:1.14.11.67, JARID1A, Jumonji/ARID domain-containing protein 1A |
计算分子量 | 192 kDa |
观测分子量 | 192 kDa |
GenBank蛋白编号 | NM_005056 |
基因名称 | KDM5A |
Gene ID (NCBI) | 5927 |
RRID | AB_10638436 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P29375 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KDM5A, also named as JARID1A, RBBP2 and RBP2, belongs to the JARID1 histone demethylase family. KDM5A is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. RBP2 may stimulate transcription mediated by nuclear receptors. It may be involved in transcriptional regulation of Hox proteins during cell differentiation. It may participate in transcriptional repression of cytokines such as CXCL12.
实验方案
Product Specific Protocols | |
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WB protocol for RBP2 antibody 18825-1-AP | Download protocol |
IF protocol for RBP2 antibody 18825-1-AP | Download protocol |
IP protocol for RBP2 antibody 18825-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
iScience Matrix stiffness aggravates osteoarthritis progression through H3K27me3 demethylation induced by mitochondrial damage | ||
Environ Toxicol JMJD3 downregulates IL4i1 aggravating lipopolysaccharide-induced acute lung injury via H3K27 and H3K4 demethylation | ||
Life Sci Treadmill exercise improves neurological function by inhibiting autophagy and the binding of HMGB1 to Beclin1 in MCAO juvenile rats. |