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  • Featured Product
  • KD/KO Validated

RBP2 Polyclonal antibody

RBP2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18825-1-AP

Print datasheet

Synonyms

KDM5A, Lysine-specific demethylase 5A, Jumonji/ARID domain-containing protein 1A, JARID1A, EC:1.14.11.67

验证数据展示

  • WB analysis of HeLa using 18825-1-AP

    WB analysis of HeLa using 18825-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18825-1-AP (RBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 18825-1-AP

    IF Staining of HeLa using 18825-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18825-1-AP (RBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HEK-293T using 18825-1-AP

    WB analysis of HEK-293T using 18825-1-AP

    WB result of KDM5A antibody (18825-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and KDM5A knockout HEK-293T cells.

  • IP experiment of HeLa using 18825-1-AP

    IP experiment of HeLa using 18825-1-AP

    IP result of anti-RBP2 (IP:18825-1-AP, 4ug; Detection:18825-1-AP 1:500) with HeLa cells lysate 1280 ug.

  • IHC staining of human stomach using 18825-1-AP

    IHC staining of human stomach using 18825-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18825-1-AP (RBP2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 18825-1-AP

    IF Staining of mouse small intestine using 18825-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 18825-1-AP

    IF Staining of mouse small intestine using 18825-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18825-1-AP targets RBP2 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysine (K)-specific demethylase 5A
Synonyms KDM5A, Lysine-specific demethylase 5A, Jumonji/ARID domain-containing protein 1A, JARID1A, EC:1.14.11.67
Calculated Molecular Weight 192 kDa
Observed Molecular Weight 192 kDa
GenBank Accession NumberNM_005056
Gene Symbol KDM5A
Gene ID (NCBI) 5927
RRIDAB_10638436
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29375
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KDM5A, also named as JARID1A, RBBP2 and RBP2, belongs to the JARID1 histone demethylase family. KDM5A is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. RBP2 may stimulate transcription mediated by nuclear receptors. It may be involved in transcriptional regulation of Hox proteins during cell differentiation. It may participate in transcriptional repression of cytokines such as CXCL12.

实验方案

Product Specific Protocols
WB protocol for RBP2 antibody 18825-1-APDownload protocol
IHC protocol for RBP2 antibody 18825-1-APDownload protocol
IF protocol for RBP2 antibody 18825-1-APDownload protocol
IP protocol for RBP2 antibody 18825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

iScience

Matrix stiffness aggravates osteoarthritis progression through H3K27me3 demethylation induced by mitochondrial damage

Authors - Tianyou Kan
View Article
mouseWB,IF

Environ Toxicol

JMJD3 downregulates IL4i1 aggravating lipopolysaccharide-induced acute lung injury via H3K27 and H3K4 demethylation

Authors - Yizhuo Gao
View Article
humanWB

Int J Biol Sci

KDM5B demethylates H3K4 to recruit XRCC1 and promote chemoresistance.

Authors - Wenxia Xu
View Article
ratIF

Life Sci

Treadmill exercise improves neurological function by inhibiting autophagy and the binding of HMGB1 to Beclin1 in MCAO juvenile rats.

Authors - Guoyuan Pan
View Article