验证数据展示
经过测试的应用
| Positive WB detected in | TF-1 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
22739-1-AP targets KDM5D in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | KDM5D fusion protein Ag18690 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lysine (K)-specific demethylase 5D |
| 别名 | H Y, Histocompatibility Y antigen, Histone demethylase JARID1D, HY, HYA, JARID1D, KDM5D, KIAA0234, lysine (K) demethylase 5D, Lysine specific demethylase 5D, Protein SmcY, SMCY |
| 计算分子量 | 1482 aa, 167 kDa |
| 观测分子量 | 70 kDa, 175 kDa |
| GenBank蛋白编号 | BC146767 |
| 基因名称 | KDM5D |
| Gene ID (NCBI) | 8284 |
| RRID | AB_2879160 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BY66 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KDM5D antibody 22739-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Androl Urol KDM5D predicts response to docetaxel chemotherapy in metastatic castration resistant prostate cancer patients. | ||
Nat Metab Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis. |