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VEGFR2/KDR Recombinant antibody, PBS Only (Capture)

VEGFR2/KDR Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230238D2

Cat no : 83049-2-PBS

Print datasheet

Synonyms

KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1

验证数据展示

  • Sandwich ELISA standard curve of MP00012-3

    Sandwich ELISA standard curve of MP00012-3

    Sandwich ELISA standard curve of MP00012-3, Human VEGFR2/KDR Recombinant Matched Antibody Pair - PBS only. 83049-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0218.83049-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP00012-3

    Sample test of MP00012-3

    HUVEC was cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for human VEGFR2/KDR, and measured 267.3 pg/mL.

  • Sample test of MP00012-3

    Sample test of MP00012-3

    The mean human VEGFR2/KDR concentration was determined to be 55,216.6 pg/mL in HUVEC cell extract based on a 0.4 mg/mL extract load.

  • Sample test of MP00012-3

    Sample test of MP00012-3

    Plasma of eight individual healthy human donors was measured. The human VEGFR2/KDR concentration of detected samples was determined to be 5,643.5 ng/mL with a range of 1,988.2 - 9,946.0 pg/mL.

  • Cytometric bead array standard curve of MP00012-1

    Cytometric bead array standard curve of MP00012-1

    Cytometric bead array standard curve of MP00012-1, KDR Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83049-2-PBS. Detection antibody: 83049-3-PBS. Standard: Eg0218. Range: 0.312-20 ng/mL.

  • Cytometric bead array standard curve of MP00012-2

    Cytometric bead array standard curve of MP00012-2

    Cytometric bead array standard curve of MP00012-2, KDR Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83049-2-PBS. Detection antibody: 83049-1-PBS. Standard: Eg0218. Range: 0.625-20 ng/mL.

产品信息

83049-2-PBS targets VEGFR2/KDR as part of a matched antibody pair:

MP00012-1: 83049-2-PBS capture and 83049-3-PBS detection (validated in Cytometric bead array)

MP00012-2: 83049-2-PBS capture and 83049-1-PBS detection (validated in Cytometric bead array)

MP00012-3: 83049-2-PBS capture and 83049-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=1.15 x 10-9M
KOff=2.30 x 10-4M
KOn=2.00 x 105M
Full Name kinase insert domain receptor (a type III receptor tyrosine kinase)
Synonyms KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1
Calculated Molecular Weight152kd
GenBank Accession NumberNM_002253
Gene Symbol KDR
Gene ID (NCBI) 3791
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35968-1
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C