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KEAP1 Polyclonal antibody, PBS Only

KEAP1 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30041-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 30041-1-AP (same clone as 30041-1-PBS)

    WB analysis using 30041-1-AP (same clone as 30041-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30041-1-AP (KEAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30041-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KEAP1 Ready-To-Use IHC Kit. KEAP1 primary antibody included.

产品信息

30041-1-PBS targets KEAP1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen KEAP1 fusion protein Ag32512 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like ECH-associated protein 1
Synonyms
Calculated Molecular Weight 624 aa, 70 kDa
Observed Molecular Weight55-70 kDa
GenBank Accession NumberBC002930
Gene Symbol KEAP1
Gene ID (NCBI) 9817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14145
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KEAP1, also named as INRF2, KIAA0132 and KLHL19, is part of a multiprotein complex that contains the CUL3-ROC1 ubiquitin ligase, which can ubiquitinate the N-terminal domain of NRF2[PMID: 20173742]. Two molecules of KEAP1 bind to two distinct sites in the N-terminal region of NRF2, the ETGE and DLG sites, which affect the KEAP1-NRF2 interaction and/or its physiological consequences[PMID: 22215675]. KEAP1 retains NFE2L2/NRF2 in the cytosol. It functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1[PMID: 20427290]. It also retains BPTF in the cytosol. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human KEAP1.