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验证数据展示

  • WB analysis using 24933-1-AP

    WB analysis using 24933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24933-1-AP (KEPI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24933-1-AP

    WB analysis of HeLa using 24933-1-AP

    WB result of KEPI antibody (24933-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KEPI transfected HeLa cells.

  • Featured Product
  • KD/KO验证

KEPI Polyclonal antibody

KEPI Polyclonal Antibody for WB, ELISA
Cat No. 24933-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

CPI17 like, KEPI, Kinase enhanced PP1 inhibitor, NY BR 81, PPP1R14C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MDA-MB-231 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24933-1-AP targets KEPI in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 KEPI fusion protein Ag20645 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 14C
别名 CPI17 like, KEPI, Kinase enhanced PP1 inhibitor, NY BR 81, PPP1R14C
计算分子量 18 kDa
观测分子量20-22 kDa
GenBank蛋白编号BC015773
基因名称 PPP1R14C
Gene ID (NCBI) 81706
RRIDAB_3085762
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAE6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R14C, found as a morphine‐regulated brain gene, encodes protein phosphatase 1 regulatory inhibitor subunit 14C, and is identified as a potent inhibitor of PP1. Horvath and colleagues reported that PPP1R14C inhibited the release of neurotransmitters and promoted neuronal exocytosis by retaining synaptosome associated protein 25 (SNAP25) at a phosphorylated state. Moreover, overexpression of PPP1R14C increased the phosphorylation of RB transcriptional co‐repressor 1 (RB1) to protect leukemic cells from chemotherapy.

实验方案

Product Specific Protocols
WB protocol for KEPI antibody 24933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols