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  • Featured Product
  • KD/KO Validated

KEPI Polyclonal antibody

KEPI Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24933-1-AP

Print datasheet

Synonyms

CPI17 like, KEPI, Kinase enhanced PP1 inhibitor, NY BR 81, PPP1R14C

验证数据展示

  • WB analysis using 24933-1-AP

    WB analysis using 24933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24933-1-AP (KEPI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24933-1-AP

    WB analysis of HeLa using 24933-1-AP

    WB result of KEPI antibody (24933-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KEPI transfected HeLa cells.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MDA-MB-231 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24933-1-AP targets KEPI in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen KEPI fusion protein Ag20645 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 14C
Synonyms CPI17 like, KEPI, Kinase enhanced PP1 inhibitor, NY BR 81, PPP1R14C
Calculated Molecular Weight 18 kDa
Observed Molecular Weight20-22 kDa
GenBank Accession NumberBC015773
Gene Symbol PPP1R14C
Gene ID (NCBI) 81706
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAE6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R14C, found as a morphine‐regulated brain gene, encodes protein phosphatase 1 regulatory inhibitor subunit 14C, and is identified as a potent inhibitor of PP1. Horvath and colleagues reported that PPP1R14C inhibited the release of neurotransmitters and promoted neuronal exocytosis by retaining synaptosome associated protein 25 (SNAP25) at a phosphorylated state. Moreover, overexpression of PPP1R14C increased the phosphorylation of RB transcriptional co‐repressor 1 (RB1) to protect leukemic cells from chemotherapy.

实验方案

Product Specific Protocols
WB protocol for KEPI antibody 24933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols