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验证数据展示

  • WB analysis of NIH/3T3 using 66265-1-Ig (same clone as 66265-1-PBS)

    WB analysis of NIH/3T3 using 66265-1-Ig (same clone as 66265-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

    IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KGA/GAC antibody included
  • IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

    IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KGA/GAC antibody included
  • IF Staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

    IF Staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66265-1-Ig (GLS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66265-1-Ig (same clone as 66265-1-PBS)

    IF Staining of HeLa using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KGA/GAC antibody (66265-1-Ig, Clone: 3A12A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KGA/GAC Monoclonal antibody, PBS Only

KGA/GAC Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66265-1-PBS

产品说明书

CloneNo. 3A12A1

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

GLS, KGA, GAM, GAC, EC:3.5.1.2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy KGA/GAC Ready-To-Use IHC Kit. KGA/GAC primary antibody included.

产品信息

66265-1-PBS targets KGA/GAC in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 KGA/GAC fusion protein Ag24227 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glutaminase
别名 GLS, KGA, GAM, GAC, EC:3.5.1.2
计算分子量 669 aa, 73 kDa
观测分子量 58 kDa, 65 kDa
GenBank蛋白编号BC038507
基因名称 GLS
Gene ID (NCBI) 2744
RRIDAB_2881651
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO94925
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.