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  • Featured Product
  • KD/KO Validated

KGA/GAC Monoclonal antibody, PBS Only

KGA/GAC Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A12A1

Cat no : 66265-1-PBS

Print datasheet

Synonyms

GLS, KGA, GAM, GAC, EC:3.5.1.2

验证数据展示

  • IF Staining of L02 using 66265-1-Ig (same clone as 66265-1-PBS)

    IF Staining of L02 using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using KGA/GAC antibody (66265-1-Ig, Clone: 3A12A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66265-1-Ig (same clone as 66265-1-PBS)

    WB analysis of NIH/3T3 using 66265-1-Ig (same clone as 66265-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

    IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KGA/GAC antibody included
  • IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

    IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KGA/GAC antibody included
  • IF Staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

    IF Staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66265-1-Ig (GLS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KGA/GAC Ready-To-Use IHC Kit. KGA/GAC primary antibody included.

产品信息

66265-1-PBS targets KGA/GAC in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen KGA/GAC fusion protein Ag24227 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutaminase
Synonyms GLS, KGA, GAM, GAC, EC:3.5.1.2
Calculated Molecular Weight 669 aa, 73 kDa
Observed Molecular Weight 58 kDa, 65 kDa
GenBank Accession NumberBC038507
Gene Symbol GLS
Gene ID (NCBI) 2744
RRIDAB_2881651
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94925
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.