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KGA-Specific Monoclonal antibody, PBS Only

KGA-Specific Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B11A11

Cat no : 66265-2-PBS

Print datasheet

Synonyms

GLS, KGA, GAM, GAC, EC:3.5.1.2

验证数据展示

  • WB analysis of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    WB analysis of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66265-2-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    IHC staining of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-2-Ig (GLS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KGA/GAC antibody included
  • IHC staining of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    IHC staining of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-2-Ig (GLS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KGA/GAC antibody included
  • IF Staining of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    IF Staining of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KGA-Specific antibody (66265-2-Ig, Clone: 1B11A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66265-2-Ig (same clone as 66265-2-PBS)

    IF Staining of A549 using 66265-2-Ig (same clone as 66265-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using KGA-Specific antibody (66265-2-Ig, Clone: 1B11A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KGA/GAC Ready-To-Use IHC Kit. KGA/GAC primary antibody included.

产品信息

66265-2-PBS targets KGA-Specific in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KGA-Specific fusion protein Ag24227 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutaminase
Synonyms GLS, KGA, GAM, GAC, EC:3.5.1.2
Calculated Molecular Weight 669 aa, 73 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC038507
Gene Symbol GLS
Gene ID (NCBI) 2744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO94925
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID: 12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID: 447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.