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验证数据展示

  • WB analysis using 31044-1-AP

    WB analysis using 31044-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 31044-1-AP (KHSRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 31044-1-AP

    IF Staining of HeLa using 31044-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (31044-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human colon using 31044-1-AP

    IHC staining of human colon using 31044-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31044-1-AP (KHSRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KHSRP Polyclonal antibody

KHSRP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31044-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KSRP, KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue
Positive IHC detected inhuman colon tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31044-1-AP targets KHSRP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen KHSRP fusion protein Ag33890 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KH-type splicing regulatory protein
Synonyms KSRP, KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2
Calculated Molecular Weight73 kDa
Observed Molecular Weight75-87kDa
GenBank Accession NumberNM_003685
Gene Symbol KHSRP
Gene ID (NCBI) 8570
RRIDAB_3669829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KHSRP antibody 31044-1-APDownload protocol
IHC protocol for KHSRP antibody 31044-1-APDownload protocol
IF protocol for KHSRP antibody 31044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols