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验证数据展示

  • WB analysis using 82931-1-RR

    WB analysis using 82931-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82931-1-RR (KHSRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 82931-1-RR

    IP experiment of HeLa using 82931-1-RR

    IP result of anti-KHSRP (IP:82931-1-RR, 4ug; Detection:82931-1-RR 1:2500) with HeLa cells lysate 1050 ug.

  • FC experiment of HeLa using 82931-1-RR

    FC experiment of HeLa using 82931-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug KHSRP Recombinant antibody (82931-1-RR, Clone:230189G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IHC staining of mouse brain using 82931-1-RR

    IHC staining of mouse brain using 82931-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82931-1-RR (KHSRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 82931-1-RR

    IF Staining of HeLa using 82931-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (82931-1-RR, Clone: 230189G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82931-1-RR

    Affinity and Kinetic Characterization of 82931-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82931-1-RR against Human KHSRP were performed. The affinity constant is 0.597 nM.

KHSRP Recombinant antibody

KHSRP Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 82931-1-RR
CloneNo. 230189G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells, mouse spleen tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82931-1-RR targets KHSRP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KHSRP fusion protein Ag33890 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name KH-type splicing regulatory protein
Synonyms KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2
Calculated Molecular Weight73 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberNM_003685
Gene Symbol KHSRP
Gene ID (NCBI) 8570
RRIDAB_3670669
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possi3bly by recruiting degradation machinery to ARE-containing mRNAs.

实验方案

Product Specific Protocols
WB protocol for KHSRP antibody 82931-1-RRDownload protocol
IHC protocol for KHSRP antibody 82931-1-RRDownload protocol
IF protocol for KHSRP antibody 82931-1-RRDownload protocol
IP protocol for KHSRP antibody 82931-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols