验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293T cells, Jurkat cells, mouse spleen tissue, mouse brain tissue, rat brain tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82931-1-RR targets KHSRP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | KHSRP fusion protein Ag33890 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | KH-type splicing regulatory protein |
Synonyms | KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2 |
Calculated Molecular Weight | 73 kDa |
Observed Molecular Weight | 75 kDa |
GenBank Accession Number | NM_003685 |
Gene Symbol | KHSRP |
Gene ID (NCBI) | 8570 |
RRID | AB_3670669 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q92945 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possi3bly by recruiting degradation machinery to ARE-containing mRNAs.
实验方案
Product Specific Protocols | |
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WB protocol for KHSRP antibody 82931-1-RR | Download protocol |
IHC protocol for KHSRP antibody 82931-1-RR | Download protocol |
IF protocol for KHSRP antibody 82931-1-RR | Download protocol |
IP protocol for KHSRP antibody 82931-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |