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验证数据展示

  • WB analysis of A549 using 25491-1-AP

    WB analysis of A549 using 25491-1-AP

    WB result of IAA0020' target='_blank'>KIAA0020 antibody (25491-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IAA0020' target='_blank'>KIAA0020 transfected A549 cells.

  • WB analysis using 25491-1-AP

    WB analysis using 25491-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25491-1-AP (PUF-A,KIAA0020 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brown disease using 25491-1-AP

    IHC staining of human brown disease using 25491-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 25491-1-AP (PUF-A,KIAA0020 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brown disease using 25491-1-AP

    IHC staining of human brown disease using 25491-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 25491-1-AP (PUF-A,KIAA0020 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

PUF-A,KIAA0020 Polyclonal antibody

PUF-A,KIAA0020 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25491-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KIAA0020, PUM3, Minor histocompatibility antigen HA-8, HLA-HA8, HBV X-transactivated gene 5 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman brown disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25491-1-AP targets PUF-A,KIAA0020 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 PUF-A,KIAA0020 fusion protein Ag22011 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA0020
别名 KIAA0020, PUM3, Minor histocompatibility antigen HA-8, HLA-HA8, HBV X-transactivated gene 5 protein
计算分子量 648 aa, 74 kDa
观测分子量 69-70 kDa
GenBank蛋白编号BC016137
基因名称 PUF-A/KIAA0020
Gene ID (NCBI) 9933
RRIDAB_2918090
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15397
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PUF-A,KIAA0020 antibody 25491-1-APDownload protocol
IHC protocol for PUF-A,KIAA0020 antibody 25491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols