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KIAA0090 Polyclonal antibody

KIAA0090 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26017-1-AP

Print datasheet

Synonyms

EMC1, ER membrane protein complex subunit 1, KIAA0090

验证数据展示

  • WB analysis of HeLa using 26017-1-AP

    WB analysis of HeLa using 26017-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26017-1-AP (KIAA0090 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 26017-1-AP

    IHC staining of human breast cancer using 26017-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26017-1-AP (KIAA0090 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26017-1-AP targets KIAA0090 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivitymouse
Immunogen KIAA0090 fusion protein Ag23406 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA0090
Synonyms EMC1, ER membrane protein complex subunit 1, KIAA0090
Observed Molecular Weight 112 kDa
GenBank Accession NumberBC034589
Gene Symbol KIAA0090
Gene ID (NCBI) 23065
RRIDAB_2880337
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N766
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIAA0090 antibody 26017-1-APDownload protocol
IHC protocol for KIAA0090 antibody 26017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

FEBS J

Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice

Authors - Xiao Li
View Article