验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HuH-7 cells, MCF-7 cells, NIH/3T3 cells, Neuro-2a cells, C6 cells |
| Positive IHC detected in | rat brain tissue, human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21016-1-AP targets KIAA0319L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | KIAA0319L fusion protein Ag15263 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA0319-like |
| 别名 | PKD1 like, KIAA1837, KIAA0319 like, Dyslexia-associated protein KIAA0319-like protein, Adeno-associated virus receptor |
| 计算分子量 | 1049 aa, 116 kDa |
| 观测分子量 | ~150 kDa |
| GenBank蛋白编号 | BC031672 |
| 基因名称 | KIAA0319L |
| Gene ID (NCBI) | 79932 |
| RRID | AB_10733523 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IZA0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIAA0319L (also known as AAVR) is a type-I transmembrane protein that acts as a receptor for adeno-associated virus (AAV) and is involved in adeno-associated virus infection through endocytosis system (PMID: 26814968). It has been shown that KIAA0319L is highly-glycosylated and can localise to the plasma membrane, endosomes, Golgi and trans-Golgi network (TGN) (PMID: 29991750). KIAA0319L gene has been associated with susceptibility to developmental dyslexia or reading disabilities (PMID: 19085271). The predicted molecular mass of KIAA0319L is 116 kDa, but western blot detected protein at 150 kDa in lysate from HeLa cells, likely due to glycosidic linkages (PMID: 28679762; 30962536).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIAA0319L antibody 21016-1-AP | Download protocol |
| IHC protocol for KIAA0319L antibody 21016-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Methods Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression | ||
Sci Adv Enhancement of liver-directed transgene expression at initial and repeat doses of AAV vectors admixed with ImmTOR nanoparticles.
| ||
Sci Rep AU040320 deficiency leads to disruption of acrosome biogenesis and infertility in homozygous mutant mice. | ||
Sci Rep Correction of LAMP3-associated salivary gland hypofunction by aquaporin gene therapy | ||
bioRxiv Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors |
