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KIAA0368 Polyclonal antibody

KIAA0368 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 25684-1-AP

Print datasheet

Synonyms

ECM29, extracellular mutant 29, KIAA0368, KIAA1962, RP11 386D8.2

验证数据展示

  • IHC staining of human liver using 25684-1-AP

    IHC staining of human liver using 25684-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25684-1-AP (KIAA0368 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human liver using 25684-1-AP

    IHC staining of human liver using 25684-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25684-1-AP (KIAA0368 antibody) at dilution of 1:200 (under 10x lens).

  • WB analysis of HEK-293 using 25684-1-AP

    WB analysis of HEK-293 using 25684-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25684-1-AP (KIAA0368 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 25684-1-AP

    IP experiment of HEK-293 using 25684-1-AP

    IP result of anti-KIAA0368 (IP:25684-1-AP, 4ug; Detection:25684-1-AP 1:600) with HEK-293 cells lysate 2600ug.

  • IF Staining of HEK-293 using 25684-1-AP

    IF Staining of HEK-293 using 25684-1-AP

    Immunofluorescent analysis of HEK-293 cells using 25684-1-AP (KIAA0368 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25684-1-AP is KIAA0368 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Tested Reactivity human
Immunogen KIAA0368 fusion protein Ag22309 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA0368
Synonyms ECM29, extracellular mutant 29, KIAA0368, KIAA1962, RP11 386D8.2
Calculated Molecular Weight 1845 aa, 204 kDa
Observed Molecular Weight 205 kDa
GenBank Accession NumberBC021127
Gene Symbol KIAA0368
Gene ID (NCBI) 23392
RRIDAB_2880193
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VYK3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ecm29 binds specifically to 26 S proteasomes. Ecm29 has been reported to stabilize proteasomes, to remodel proteasomes upon stress, to be involved in membrane-associated localization of proteasomes, and, more recently, to be involved in quality control or assembly of the proteasome. Ecm29 acts as a proteasome inhibitor.

实验方案

Product Specific Protocols
WB protocol for KIAA0368 antibody 25684-1-APDownload protocol
IHC protocol for KIAA0368 antibody 25684-1-APDownload protocol
IF protocol for KIAA0368 antibody 25684-1-APDownload protocol
IP protocol for KIAA0368 antibody 25684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols