MRPP3 Polyclonal antibody
MRPP3 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, L02 cells, mouse liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
20959-1-AP targets MRPP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | MRPP3 fusion protein Ag15196 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | KIAA0391 |
| Synonyms | KIAA0391, Protein only RNase P catalytic subunit, PRORP, Mitochondrial ribonuclease P catalytic subunit, EC:3.1.26.5 |
| Calculated Molecular Weight | 583 aa, 67 kDa |
| Observed Molecular Weight | 65-67 kDa |
| GenBank Accession Number | BC032221 |
| Gene Symbol | MRPP3 |
| Gene ID (NCBI) | 9692 |
| RRID | AB_10860109 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15091 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MRPP3 (Mitochondrial ribonuclease P protein 3), originally known as KIAA0391, has been identified as a member of human mitochondrial RNase P (mtRNase P), the endonuclease responsible for tRNA 5' end maturation by removing extra nucleotides from tRNA precursors at their 5' termini. Recently Daniel et al. reported that Rnase P and other mitochondrial RNA processing enzymes are localized near mt DNA nucleoids where they can initiate process of primary transcripts. (24703694)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPP3 antibody 20959-1-AP | Download protocol |
| IF protocol for MRPP3 antibody 20959-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids. |
