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KIAA0513 Polyclonal antibody

KIAA0513 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21478-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis of mouse brain using 21478-1-AP

    WB analysis of mouse brain using 21478-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21478-1-AP

    IF Staining of HepG2 using 21478-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIAA0513 antibody 21478-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human ovary cancer using 21478-1-AP

    IHC staining of human ovary cancer using 21478-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21478-1-AP (KIAA0513 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 21478-1-AP

    IHC staining of human ovary cancer using 21478-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21478-1-AP (KIAA0513 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, human testis tissue, mouse cerebellum tissue, mouse kidney tissue, mouse testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21478-1-AP targets KIAA0513 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KIAA0513 fusion protein Ag15624 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA0513
Synonyms
Calculated Molecular Weight 411 aa, 47 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC030280
Gene Symbol KIAA0513
Gene ID (NCBI) 9764
RRIDAB_10755300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60268
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA0513 was identified as upregulated in subjects with schizophrenia. The mRNA of KIAA0513 is ubiquitously expressed but is enriched in the brain, particularly in the cerebellum. The interacting protein analysis indicated that KIAA0513 is involved in both synaptic and apoptotic signaling. (PMID: 17010949) Several isoforms of KIAA0513 (47/46/34 kDa) exist due to the alternative splicing. This antibody is expected to recognize all of isoforms of KIAA0513.

实验方案

Product Specific Protocols
WB protocol for KIAA0513 antibody 21478-1-APDownload protocol
IHC protocol for KIAA0513 antibody 21478-1-APDownload protocol
IF protocol for KIAA0513 antibody 21478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols