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KIAA0528 Polyclonal antibody

KIAA0528 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21138-1-AP

Print datasheet

Synonyms

CDP138, C2CD5, C2 domain-containing protein 5, C2 domain-containing phosphoprotein of 138 kDa

验证数据展示

  • WB analysis using 21138-1-AP

    WB analysis using 21138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 21138-1-AP

    IHC staining of mouse small intestine using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 21138-1-AP

    IHC staining of mouse small intestine using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 21138-1-AP

    IF Staining of HepG2 using 21138-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIAA0528 antibody 21138-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HeLa using 21138-1-AP

    IP experiment of HeLa using 21138-1-AP

    IP result of anti-KIAA0528 (IP:21138-1-AP, 4ug; Detection:21138-1-AP 1:700) with HeLa cells lysate 520ug.

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21138-1-AP targets KIAA0528 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KIAA0528 fusion protein Ag15590 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA0528
Synonyms CDP138, C2CD5, C2 domain-containing protein 5, C2 domain-containing phosphoprotein of 138 kDa
Calculated Molecular Weight 1000 aa, 110 kDa
Observed Molecular Weight 110-125 kDa
GenBank Accession NumberBC042498
Gene Symbol KIAA0528
Gene ID (NCBI) 9847
RRIDAB_10858480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86YS7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIAA0528 antibody 21138-1-APDownload protocol
IHC protocol for KIAA0528 antibody 21138-1-APDownload protocol
IF protocol for KIAA0528 antibody 21138-1-APDownload protocol
IP protocol for KIAA0528 antibody 21138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols