• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82718-3-RR

    WB analysis using 82718-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82718-3-RR (KIAA0652 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82718-3-RR

    WB analysis of HEK-293 using 82718-3-RR

    WB result of ATG13 antibody (82718-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG13 transfected HEK-293 cells.

  • IHC staining of human ovarian cancer using 82718-3-RR

    IHC staining of human ovarian cancer using 82718-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82718-3-RR (ATG13 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82718-3-RR

    Affinity and Kinetic Characterization of 82718-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82718-3-RR against Human ATG13 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

ATG13 Recombinant antibody

ATG13 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 82718-3-RR

产品说明书

CloneNo. 3G14

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

KIAA0652, 3G14, Autophagy related protein 13, Autophagy-related protein 13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HEK- 293 cells, BGC-823 cells, SH-SY5Y cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82718-3-RR targets ATG13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATG13 fusion protein Ag13090 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 KIAA0652
别名 KIAA0652, 3G14, Autophagy related protein 13, Autophagy-related protein 13
计算分子量 57 kDa
观测分子量70 kDa
GenBank蛋白编号BC001331
基因名称 ATG13
Gene ID (NCBI) 9776
RRIDAB_3086517
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75143
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

实验方案

Product Specific Protocols
WB protocol for ATG13 antibody 82718-3-RRDownload protocol
IHC protocol for ATG13 antibody 82718-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols