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ATG13 Recombinant antibody

ATG13 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G14

Cat no : 82718-3-RR

Print datasheet

Synonyms

KIAA0652, Autophagy-related protein 13, Autophagy related protein 13, 3G14

验证数据展示

  • WB analysis using 82718-3-RR

    WB analysis using 82718-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82718-3-RR (KIAA0652 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82718-3-RR

    WB analysis of HEK-293 using 82718-3-RR

    WB result of ATG13 antibody (82718-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG13 transfected HEK-293 cells.

  • IHC staining of human ovarian cancer using 82718-3-RR

    IHC staining of human ovarian cancer using 82718-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82718-3-RR (ATG13 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82718-3-RR

    Affinity and Kinetic Characterization of 82718-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82718-3-RR against Human ATG13 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HEK- 293 cells, BGC-823 cells, SH-SY5Y cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82718-3-RR targets ATG13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATG13 fusion protein Ag13090 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name KIAA0652
Synonyms KIAA0652, Autophagy-related protein 13, Autophagy related protein 13, 3G14
Calculated Molecular Weight 57 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC001331
Gene Symbol ATG13
Gene ID (NCBI) 9776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75143
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

实验方案

Product Specific Protocols
WB protocol for ATG13 antibody 82718-3-RRDownload protocol
IHC protocol for ATG13 antibody 82718-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols