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验证数据展示

  • IF Staining of A431 using 23360-1-AP

    IF Staining of A431 using 23360-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using KIAA0922 antibody (23360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse colon using 23360-1-AP

    IHC staining of mouse colon using 23360-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23360-1-AP (KIAA0922 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KIAA0922 Polyclonal antibody

KIAA0922 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 23360-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

KIAA0922, TMEM131L, Transmembrane protein 131 like

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, U-251 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23360-1-AP targets KIAA0922 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 KIAA0922 fusion protein Ag19898 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA0922
别名 KIAA0922, TMEM131L, Transmembrane protein 131 like
计算分子量 1609 aa, 179 kDa
GenBank蛋白编号BC131505
基因名称 KIAA0922
Gene ID (NCBI) 23240
RRIDAB_3085709
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA2VDJ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA0922 is also named as TMEM131L (Transmembrane protein 131-like). Human KIAA0922/TMEM131L encodes a transmembrane protein, TMEM131L, that regulates the canonical Wnt/β-catenin signaling pathway by eliciting the lysosome-dependent degradation of phosphorylated LRP6 co-receptor (PMID: 28347816).

实验方案

Product Specific Protocols
IHC protocol for KIAA0922 antibody 23360-1-APDownload protocol
IF protocol for KIAA0922 antibody 23360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols