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  • Featured Product
  • KD/KO Validated

KIAA1191 Polyclonal antibody

KIAA1191 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20637-1-AP

Print datasheet

Synonyms

Putative monooxygenase p33MONOX, P33MONOX, Flavin monooxygenase motif-containing protein of 33 kDa, EC:1.-.-.-

验证数据展示

  • WB analysis of mouse ovary using 20637-1-AP

    WB analysis of mouse ovary using 20637-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20637-1-AP (KIAA1191 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20637-1-AP

    IF Staining of HeLa using 20637-1-AP

    Immunofluorescent analysis of Hela cells, using KIAA1191 antibody 20637-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human stomach using 20637-1-AP

    IHC staining of human stomach using 20637-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20637-1-AP (KIAA1191 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse ovary tissue, mouse uterus tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20637-1-AP targets KIAA1191 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen KIAA1191 fusion protein Ag14694 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1191
Synonyms Putative monooxygenase p33MONOX, P33MONOX, Flavin monooxygenase motif-containing protein of 33 kDa, EC:1.-.-.-
Calculated Molecular Weight 305 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC006316
Gene Symbol KIAA1191
Gene ID (NCBI) 57179
RRIDAB_10693625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96A73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIAA1191 antibody 20637-1-APDownload protocol
IHC protocol for KIAA1191 antibody 20637-1-APDownload protocol
IF protocol for KIAA1191 antibody 20637-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Ann Hematol

The role of KIAA1191 in the necroptotic pathway of multiple myeloma.

Authors - Zhao Xu
  • KD Validated
View Article