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验证数据展示

  • WB analysis using 31106-1-AP

    WB analysis using 31106-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31106-1-AP (KIAA1199 / CEMIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 31106-1-AP

    IHC staining of human colon cancer using 31106-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 31106-1-AP (KIAA1199 / CEMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 31106-1-AP

    IF Staining of HeLa using 31106-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KIAA1199 / CEMIP antibody (31106-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

KIAA1199 / CEMIP Polyclonal antibody

KIAA1199 / CEMIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31106-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA1199, CEMIP, Protein KIAA1199, HYBID, Hyaluronan binding protein involved in hyaluronan depolymerization

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HT-1080 cells
Positive IHC detected inhuman colon cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31106-1-AP targets KIAA1199 / CEMIP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 KIAA1199 / CEMIP fusion protein Ag34315 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1199 / CEMIP
别名 KIAA1199, CEMIP, Protein KIAA1199, HYBID, Hyaluronan binding protein involved in hyaluronan depolymerization
计算分子量 1361 aa, 153 kDa
观测分子量150 kDa
GenBank蛋白编号BC020256
基因名称 KIAA1199
Gene ID (NCBI) 57214
RRIDAB_3669858
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8WUJ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein whose genetic mutations were linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression.

实验方案

Product Specific Protocols
WB protocol for KIAA1199 / CEMIP antibody 31106-1-APDownload protocol
IHC protocol for KIAA1199 / CEMIP antibody 31106-1-APDownload protocol
IF protocol for KIAA1199 / CEMIP antibody 31106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols