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验证数据展示

  • WB analysis using 31106-1-AP

    WB analysis using 31106-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31106-1-AP (KIAA1199 / CEMIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 31106-1-AP

    IHC staining of human colon cancer using 31106-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 31106-1-AP (KIAA1199 / CEMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 31106-1-AP

    IF Staining of HeLa using 31106-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KIAA1199 / CEMIP antibody (31106-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

KIAA1199 / CEMIP Polyclonal antibody

KIAA1199 / CEMIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31106-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA1199, CEMIP, Protein KIAA1199, HYBID, Hyaluronan binding protein involved in hyaluronan depolymerization

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HT-1080 cells
Positive IHC detected inhuman colon cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31106-1-AP targets KIAA1199 / CEMIP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen KIAA1199 / CEMIP fusion protein Ag34315 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1199 / CEMIP
Synonyms KIAA1199, CEMIP, Protein KIAA1199, HYBID, Hyaluronan binding protein involved in hyaluronan depolymerization
Calculated Molecular Weight 1361 aa, 153 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC020256
Gene Symbol KIAA1199
Gene ID (NCBI) 57214
RRIDAB_3669858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8WUJ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein whose genetic mutations were linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression.

实验方案

Product Specific Protocols
WB protocol for KIAA1199 / CEMIP antibody 31106-1-APDownload protocol
IHC protocol for KIAA1199 / CEMIP antibody 31106-1-APDownload protocol
IF protocol for KIAA1199 / CEMIP antibody 31106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols