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KIAA1217 Polyclonal antibody

KIAA1217 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24880-1-AP

Print datasheet

Synonyms

KIAA1217, Sickle tail protein homolog, SKT

验证数据展示

  • WB analysis using 24880-1-AP

    WB analysis using 24880-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 24880-1-AP

    WB analysis using 24880-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, rat colon tissue, mouse colon tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24880-1-AP targets KIAA1217 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KIAA1217 fusion protein Ag19367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1217
Synonyms KIAA1217, Sickle tail protein homolog, SKT
Calculated Molecular Weight 1943 aa, 214 kDa
Observed Molecular Weight100-110 kDa, 140-160 kDa, 210 kDa
GenBank Accession NumberBC018764
Gene Symbol KIAA1217
Gene ID (NCBI) 56243
RRIDAB_2879775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T5P2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1217, sickle tail protein homolog, is required for normal development of intervertebral disks. It promotes epithelial-mesenchymal transition and hepatocellular carcinoma metastasis by interacting with and activating STAT3, indicating its potential as a therapeutic target for curbing HCC metastasis (PMID: 35008530). It is also a novel candidate gene associated with isolated and syndromic vertebral malformations (PMID: 32369272). KIAA1217 has 9 alternatively spliced isoforms of 100-110 kDa, 140-160 kDa, 210 kDa.

实验方案

Product Specific Protocols
WB protocol for KIAA1217 antibody 24880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols