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  • Featured Product
  • KD/KO Validated

KIAA1217 Recombinant antibody

KIAA1217 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240276F3

Cat no : 83361-2-RR

Print datasheet

Synonyms

SKT, Sickle tail protein homolog, 240276F3

验证数据展示

  • WB analysis using 83361-2-RR

    WB analysis using 83361-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83361-2-RR (KIAA1217 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 83361-2-RR

    WB analysis of HEK-293 using 83361-2-RR

    WB result of IAA1217' target='_blank'>KIAA1217 antibody (83361-2-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IAA1217' target='_blank'>KIAA1217 transfected HEK-293 cells.

  • FC experiment of MCF-7 using 83361-2-RR

    FC experiment of MCF-7 using 83361-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug KIAA1217 Recombinant antibody (83361-2-RR, Clone:240276F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83361-2-RR

    Affinity and Kinetic Characterization of 83361-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83361-2-RR against Human KIAA1217 were performed. The affinity constant is 1.46 nM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83361-2-RR targets KIAA1217 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen KIAA1217 fusion protein Ag19367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name KIAA1217
Synonyms SKT, Sickle tail protein homolog, 240276F3
Calculated Molecular Weight 1943 aa, 214 kDa
Observed Molecular Weight100-110, 140-160, 210
GenBank Accession NumberBC018764
Gene Symbol KIAA1217
Gene ID (NCBI) 56243
RRIDAB_3671015
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5T5P2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1217, sickle tail protein homolog, is required for normal development of intervertebral disks. It promotes epithelial-mesenchymal transition and hepatocellular carcinoma metastasis by interacting with and activating STAT3, indicating its potential as a therapeutic target for curbing HCC metastasis (PMID: 35008530). It is also a novel candidate gene associated with isolated and syndromic vertebral malformations (PMID: 32369272). KIAA1217 has 9 alternatively spliced isoforms of 100-110 kDa, 140-160 kDa, 210 kDa.

实验方案

Product Specific Protocols
WB protocol for KIAA1217 antibody 83361-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols