验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, K-562 cells, MDA-MB-231 cells, SKOV-3 cells |
Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 1 publications below |
产品信息
30166-1-AP targets VIRMA/KIAA1429 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | VIRMA/KIAA1429 fusion protein Ag32830 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | KIAA1429 |
Synonyms | KIAA1429, VIRMA, MSTP054 |
Observed Molecular Weight | 200-210 kDa |
GenBank Accession Number | BC003701 |
Gene Symbol | KIAA1429 |
Gene ID (NCBI) | 25962 |
RRID | AB_2935521 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q69YN4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. Moreover, this antibody can not recognize short isoforms.
实验方案
Product Specific Protocols | |
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WB protocol for VIRMA/KIAA1429 antibody 30166-1-AP | Download protocol |
IHC protocol for VIRMA/KIAA1429 antibody 30166-1-AP | Download protocol |
IF protocol for VIRMA/KIAA1429 antibody 30166-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Lett KIAA1429 facilitates metastasis via m6A-YTHDC1-dependent RND3 down-regulation in hepatocellular carcinoma cells
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BMC Biol The m6A writer KIAA1429 regulates photoaging progression via MFAP4-dependent collagen synthesis |