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KIAA1468 Polyclonal antibody

KIAA1468 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18993-1-AP

Print datasheet

Synonyms

HsT3308, HsT885, KIAA1468, RAB11-binding protein RELCH, RELCH

验证数据展示

  • WB analysis using 18993-1-AP

    WB analysis using 18993-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18993-1-AP (KIAA1468 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 18993-1-AP

    IP experiment of mouse brain using 18993-1-AP

    IP result of anti-KIAA1468 (IP:18993-1-AP, 4ug; Detection:18993-1-AP 1:500) with mouse brain tissue lysate 1280 ug.

  • IHC staining of mouse kidney using 18993-1-AP

    IHC staining of mouse kidney using 18993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18993-1-AP (KIAA1468 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 18993-1-AP

    IHC staining of mouse kidney using 18993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18993-1-AP (KIAA1468 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18993-1-AP targets KIAA1468 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1468
Synonyms HsT3308, HsT885, KIAA1468, RAB11-binding protein RELCH, RELCH
Calculated Molecular Weight 139 kDa
Observed Molecular Weight 100-135 kDa
GenBank Accession NumberNM_020854
Gene Symbol KIAA1468
Gene ID (NCBI) 57614
RRIDAB_10598164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P260
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RELCH/KIAA1468, which is a newly identified Rab11 effector protein, tethers the recycling endosomes (REs) and trans-Golgi network (TGN) membranes by binding TGN-localized oxysterol-binding protein (OSBP) and promotes OSBP-dependent nonvesicular cholesterol transport from REs to the TGN.

实验方案

Product Specific Protocols
WB protocol for KIAA1468 antibody 18993-1-APDownload protocol
IHC protocol for KIAA1468 antibody 18993-1-APDownload protocol
IP protocol for KIAA1468 antibody 18993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols