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  • KD/KO Validated

CEP44 Polyclonal antibody

CEP44 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 24457-1-AP

Print datasheet

Synonyms

PS1TP3, HBV PreS1-transactivated protein 3, Centrosomal protein of 44 kDa



经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue, Jurkat cells, MCF-7 cells
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24457-1-AP targets CEP44 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen CEP44 fusion protein Ag19961 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1712
Synonyms PS1TP3, HBV PreS1-transactivated protein 3, Centrosomal protein of 44 kDa
Calculated Molecular Weight 390 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC038667
Gene Symbol CEP44
Gene ID (NCBI) 80817
RRIDAB_2879557
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C0F1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP44, also named as KIAA1712 and PS1TP3, localizes to centrosomes, spindle poles, and weakly in midbody. It is a 44 kDa centrosome protein.

实验方案

Product Specific Protocols
WB protocol for CEP44 antibody 24457-1-APDownload protocol
IHC protocol for CEP44 antibody 24457-1-APDownload protocol
IF protocol for CEP44 antibody 24457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Cell Biol

Centrosomal organization of Cep152 provides flexibility in Plk4 and procentriole positioning

Authors - Catherine Sullenberger
humanWB,IF,IP

J Cell Sci

Cep44 functions in centrosome cohesion by stabilizing rootletin

Authors - Delowar Hossain
  • KD Validated
humanWB,IF

J Cell Sci

Cep44 functions in centrosome cohesion by stabilizing rootletin.

Authors - Delowar Hossain
  • KD Validated

Mol Biol Cell

"Roles for ELMOD2 and Rootletin in Ciliogenesis".

Authors - Rachel E Turn
mouseWB

Cell Rep

A program of successive gene expression in mouse one-cell embryos

Authors - Maki Asami
humanIF

Nat Commun

Molecular basis promoting centriole triplet microtubule assembly

Authors - Yutaka Takeda