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KIAA1715 Polyclonal antibody

KIAA1715 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30857-1-AP

Print datasheet

Synonyms

DKFZp686G1442, KIAA1715, LNP, Protein lunapark, Ul, ulnaless

验证数据展示

  • WB analysis using 30857-1-AP

    WB analysis using 30857-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30857-1-AP (KIAA1715 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 30857-1-AP

    IHC staining of mouse cerebellum using 30857-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30857-1-AP (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells, K-562 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30857-1-AP targets KIAA1715 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen KIAA1715 fusion protein Ag33996 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1715
Synonyms DKFZp686G1442, KIAA1715, LNP, Protein lunapark, Ul, ulnaless
Observed Molecular Weight53 kDa
GenBank Accession NumberBC105132
Gene Symbol KIAA1715
Gene ID (NCBI) 80856
RRIDAB_3086424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9C0E8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

实验方案

Product Specific Protocols
WB protocol for KIAA1715 antibody 30857-1-APDownload protocol
IHC protocol for KIAA1715 antibody 30857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols