• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

KIAA1715 Recombinant antibody

KIAA1715 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240211B9

Cat no : 83418-5-RR

Print datasheet

Synonyms

LNP, Ul, Protein lunapark, LNPK, ER junction formation factor lunapark

验证数据展示

  • WB analysis using 83418-5-RR

    WB analysis using 83418-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83418-5-RR (KIAA1715 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 83418-5-RR

    IF Staining of U-251 using 83418-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using KIAA1715 antibody (83418-5-RR, Clone: 240211B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse cerebellum using 83418-5-RR

    IHC staining of mouse cerebellum using 83418-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83418-5-RR (KIAA1715 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, HepG2 cells, U2OS cells, mouse testis tissue, mouse colon tissue, mouse kidney tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83418-5-RR targets KIAA1715 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen KIAA1715 fusion protein Ag34669 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name KIAA1715
Synonyms LNP, Ul, Protein lunapark, LNPK, ER junction formation factor lunapark
Observed Molecular Weight53-55 kDa
GenBank Accession NumberBC105132
Gene Symbol KIAA1715
Gene ID (NCBI) 80856
RRIDAB_3671067
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9C0E8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

实验方案

Product Specific Protocols
WB protocol for KIAA1715 antibody 83418-5-RRDownload protocol
IHC protocol for KIAA1715 antibody 83418-5-RRDownload protocol
IF protocol for KIAA1715 antibody 83418-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols