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验证数据展示

  • WB analysis of HeLa using 24395-1-AP

    WB analysis of HeLa using 24395-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24395-1-AP (MLK4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of C6 using 24395-1-AP

    IF Staining of C6 using 24395-1-AP

    Immunofluorescent analysis of C6 cells using 24395-1-AP (MLK4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

MLK4 Polyclonal antibody

MLK4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24395-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

KIAA1804, mixed lineage kinase 4, MLK4, RP5 862P8.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inC6 cells
Planning an IHC experiment? We recommend our IHCeasy MLK4 Ready-To-Use IHC Kit. MLK4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24395-1-AP targets MLK4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MLK4 fusion protein Ag18904 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mixed lineage kinase 4
别名 KIAA1804, mixed lineage kinase 4, MLK4, RP5 862P8.2
计算分子量 1036 aa, 114 kDa
观测分子量 53 kDa
GenBank蛋白编号BC136649
基因名称 MLK4
Gene ID (NCBI) 84451
RRIDAB_2879522
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5TCX8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MLK4 belongs to the MAP kinase kinase kinase subfamily. The MLK family members are characterized by the presence of signature sequences ofSer/Thr and Tyr kinases within their catalytic domain. The MLK1-4 contains an aminoterminal Src-Homology (SH3) domain, followed by the kinase domain, a leucine-zipper region, and a Cdc42/Rac-interactive binding (CRIB) motif. MLK4 expressed in two splicing variants, MLK4α and MLK4β. The difference of these two isoforms is located in their C-terminal portion. The negative effect of MLK4 on LPS-induced ERK and JNK activation could be very important in controlling the level of cytokine expression, and thus maintaining the balance of inflammatory responses.(PMID:21602844). This protein has 3 isoforms produced by alternative splicing with the molecular weight of 63 kDa, 113 kDa, 53 kDa.

实验方案

Product Specific Protocols
WB protocol for MLK4 antibody 24395-1-APDownload protocol
IF protocol for MLK4 antibody 24395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols