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MLK4 Polyclonal antibody

MLK4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24395-1-AP

Print datasheet

Synonyms

KIAA1804, mixed lineage kinase 4, MLK4, RP5 862P8.2

验证数据展示

  • WB analysis of HeLa using 24395-1-AP

    WB analysis of HeLa using 24395-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24395-1-AP (MLK4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of C6 using 24395-1-AP

    IF Staining of C6 using 24395-1-AP

    Immunofluorescent analysis of C6 cells using 24395-1-AP (MLK4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inC6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24395-1-AP is MLK4 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MLK4 fusion protein Ag18904 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mixed lineage kinase 4
Synonyms KIAA1804, mixed lineage kinase 4, MLK4, RP5 862P8.2
Calculated Molecular Weight 1036 aa, 114 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC136649
Gene Symbol MLK4
Gene ID (NCBI) 84451
RRIDAB_2879522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5TCX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MLK4 belongs to the MAP kinase kinase kinase subfamily. The MLK family members are characterized by the presence of signature sequences ofSer/Thr and Tyr kinases within their catalytic domain. The MLK1-4 contains an aminoterminal Src-Homology (SH3) domain, followed by the kinase domain, a leucine-zipper region, and a Cdc42/Rac-interactive binding (CRIB) motif. MLK4 expressed in two splicing variants, MLK4α and MLK4β. The difference of these two isoforms is located in their C-terminal portion. The negative effect of MLK4 on LPS-induced ERK and JNK activation could be very important in controlling the level of cytokine expression, and thus maintaining the balance of inflammatory responses.(PMID:21602844). This protein has 3 isoforms produced by alternative splicing with the molecular weight of 63 kDa, 113 kDa, 53 kDa.

实验方案

Product Specific Protocols
WB protocol for MLK4 antibody 24395-1-APDownload protocol
IF protocol for MLK4 antibody 24395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols