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KIF13B Polyclonal antibody

KIF13B Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 30141-1-AP

Print datasheet

Synonyms

kinesin family member 13B, KIAA0639, GAKIN

验证数据展示

  • WB analysis using 30141-1-AP

    WB analysis using 30141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30141-1-AP (KIF13B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 30141-1-AP

    IP experiment of HEK-293 using 30141-1-AP

    IP result of anti-KIF13B (IP:30141-1-AP, 4ug; Detection:30141-1-AP 1:800) with HEK-293 cells lysate 1400 ug.

  • IHC staining of human stomach cancer using 30141-1-AP

    IHC staining of human stomach cancer using 30141-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30141-1-AP (KIF13B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 30141-1-AP

    IHC staining of human stomach cancer using 30141-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30141-1-AP (KIF13B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30141-1-AP targets KIF13B in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human
Immunogen KIF13B fusion protein Ag30876 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 13B
Synonyms kinesin family member 13B, KIAA0639, GAKIN
Calculated Molecular Weight 203 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberNM_015254
Gene Symbol KIF13B
Gene ID (NCBI) 23303
RRIDAB_3086245
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQT8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF13B, Also known as GAKIN, is a 250-kD phosphoprotein. KIF13B contains a consensus ATP/GTP-binding motif within its N-terminal motor domain; a stalk domain containing several short alpha-helical segments; and a CAP-gly motif in its C terminus characteristic of microtubule-based cytoskeleton components(PMID: 10859302).

实验方案

Product Specific Protocols
WB protocol for KIF13B antibody 30141-1-APDownload protocol
IHC protocol for KIF13B antibody 30141-1-APDownload protocol
IP protocol for KIF13B antibody 30141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols