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验证数据展示

  • WB analysis using 30774-1-AP

    WB analysis using 30774-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30774-1-AP (KIF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

KIF15 Polyclonal antibody

KIF15 Polyclonal Antibody for WB, ELISA
Cat No. 30774-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

FLJ25667, HKLP2, KIF15, kinesin family member 15, Kinesin like protein 2, Kinesin like protein 7, Kinesin like protein KIF15, KLP2, KNSL7, NY BR 62

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cels, K-562 cells, L02 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30774-1-AP targets KIF15 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 KIF15 fusion protein Ag33897 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 15
别名 FLJ25667, HKLP2, KIF15, kinesin family member 15, Kinesin like protein 2, Kinesin like protein 7, Kinesin like protein KIF15, KLP2, KNSL7, NY BR 62
计算分子量160 kDa
观测分子量145-150 kDa
GenBank蛋白编号NM_020242
基因名称 KIF15
Gene ID (NCBI) 56992
RRIDAB_3086416
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NS87
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. It is located in the cytoplasm and biased expression in testis. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15 (14618103). It is known that KIF15 participates in important events during neuronal development and involves multiple cellular processes such as proliferation, apoptosis, and differentiation. There is emerging evidence indicating that KIF15 plays an important role in several malignancies including pancreatic cancer, hepatocellular carcinoma, and breast cancer (PMID: 33277366).

实验方案

Product Specific Protocols
WB protocol for KIF15 antibody 30774-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols