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验证数据展示

  • WB analysis of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

    WB analysis of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

    WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HEK-293 cells. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 55407-1-AP (same clone as 55407-1-PBS)

    WB analysis of Jurkat using 55407-1-AP (same clone as 55407-1-PBS)

    WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected Jurkat cells. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • WB analysis using 55407-1-AP (same clone as 55407-1-PBS)

    WB analysis using 55407-1-AP (same clone as 55407-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 55407-1-AP (same clone as 55407-1-PBS)

    IHC staining of human testis using 55407-1-AP (same clone as 55407-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 55407-1-AP (same clone as 55407-1-PBS)

    IHC staining of human testis using 55407-1-AP (same clone as 55407-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse thymus using 55407-1-AP (same clone as 55407-1-PBS)

    IP experiment of mouse thymus using 55407-1-AP (same clone as 55407-1-PBS)

    IP result of anti-KIF15 (IP:55407-1-AP, 4ug; Detection:55407-1-AP 1:500) with mouse thymus tissue lysate 2800ug. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

    FC experiment of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug KIF15 Polyclonal antibody (55407-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KIF15 Polyclonal antibody, PBS Only

KIF15 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 55407-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

KNSL7, KLP2, Kinesin-like protein KIF15, Kinesin-like protein 7, Kinesin-like protein 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

55407-1-PBS targets KIF15 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 15
别名 KNSL7, KLP2, Kinesin-like protein KIF15, Kinesin-like protein 7, Kinesin-like protein 2
计算分子量 160 kDa
观测分子量 150-165 kDa
GenBank蛋白编号NM_020242
基因名称 KIF15
Gene ID (NCBI) 56992
RRIDAB_11182836
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NS87
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)