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  • Featured Product
  • KD/KO Validated

KIF15 Polyclonal antibody, PBS Only

KIF15 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55407-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

    WB analysis of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

    WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HEK-293 cells. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 55407-1-AP (same clone as 55407-1-PBS)

    WB analysis of Jurkat using 55407-1-AP (same clone as 55407-1-PBS)

    WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected Jurkat cells. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • WB analysis using 55407-1-AP (same clone as 55407-1-PBS)

    WB analysis using 55407-1-AP (same clone as 55407-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 55407-1-AP (same clone as 55407-1-PBS)

    IHC staining of human testis using 55407-1-AP (same clone as 55407-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 55407-1-AP (same clone as 55407-1-PBS)

    IHC staining of human testis using 55407-1-AP (same clone as 55407-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse thymus using 55407-1-AP (same clone as 55407-1-PBS)

    IP experiment of mouse thymus using 55407-1-AP (same clone as 55407-1-PBS)

    IP result of anti-KIF15 (IP:55407-1-AP, 4ug; Detection:55407-1-AP 1:500) with mouse thymus tissue lysate 2800ug. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

    IF Staining of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 55407-1-AP (KIF15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

    FC experiment of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug KIF15 Polyclonal antibody (55407-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

产品信息

55407-1-PBS targets KIF15 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 15
Synonyms
Calculated Molecular Weight 160 kDa
Observed Molecular Weight 150-165 kDa
GenBank Accession NumberNM_020242
Gene Symbol KIF15
Gene ID (NCBI) 56992
RRIDAB_11182836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NS87
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)