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  • Featured Product
  • KD/KO Validated

KIF17 Polyclonal antibody

KIF17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14615-1-AP

Print datasheet

Synonyms

Kinesin-like protein KIF17, KIF3X, KIF3-related motor protein, KIAA1405

验证数据展示

  • IP experiment of mouse brain using 14615-1-AP

    IP experiment of mouse brain using 14615-1-AP

    IP result of anti-KIF17 (IP:14615-1-AP, 4ug; Detection:14615-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of human gliomas using 14615-1-AP

    IHC staining of human gliomas using 14615-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14615-1-AP (KIF17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 14615-1-AP

    IHC staining of human gliomas using 14615-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14615-1-AP (KIF17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of COS-7 using 14615-1-AP

    IF Staining of COS-7 using 14615-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed COS-7 cells using 14615-1-AP (KIF17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of COS-7 using 14615-1-AP

    IF Staining of COS-7 using 14615-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed COS-7 cells using KIF17 antibody (14615-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis using 14615-1-AP

    WB analysis using 14615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14615-1-AP (KIF17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOS-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14615-1-AP targets KIF17 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse
Immunogen KIF17 fusion protein Ag6217 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 17
Synonyms Kinesin-like protein KIF17, KIF3X, KIF3-related motor protein, KIAA1405
Calculated Molecular Weight 115 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC065927
Gene Symbol KIF17
Gene ID (NCBI) 57576
RRIDAB_2131278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2E2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF17 antibody 14615-1-APDownload protocol
IHC protocol for KIF17 antibody 14615-1-APDownload protocol
IF protocol for KIF17 antibody 14615-1-APDownload protocol
IP protocol for KIF17 antibody 14615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Dev Cell

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Authors - Jie Ran
View Article
humanWB,IF,IP

Neurosci Bull

KIF17 Modulates Epileptic Seizures and Membrane Expression of the NMDA Receptor Subunit NR2B.

Authors - Yan Liu
View Article
humanWB,IHC,IF,CoIP

Cancer Lett

KIF17 maintains the epithelial phenotype of breast cancer cells and curbs tumour metastasis

Authors - Tianfu Li
  • KD Validated
View Article