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验证数据展示

  • WB analysis using 85046-1-RR

    WB analysis using 85046-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85046-1-RR (KIF17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 85046-1-RR

    IHC staining of mouse brain using 85046-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85046-1-RR (KIF17 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85046-1-RR

    Affinity and Kinetic Characterization of 85046-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85046-1-RR against Human KIF17 were performed. The affinity constant is below 1 pM.

KIF17 Recombinant antibody

KIF17 Recombinant Antibody for WB, IHC, ELISA
Cat No. 85046-1-RR

产品说明书

CloneNo. 242469G4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Kinesin-like protein KIF17, KIF3X, KIF3-related motor protein, KIAA1405, 242469G4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85046-1-RR targets KIF17 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KIF17 fusion protein Ag6217 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kinesin family member 17
别名 Kinesin-like protein KIF17, KIF3X, KIF3-related motor protein, KIAA1405, 242469G4
计算分子量 115 kDa
观测分子量 170 kDa
GenBank蛋白编号BC065927
基因名称 KIF17
Gene ID (NCBI) 57576
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9P2E2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF17 is a kinesin superfamily member, encoding a neuron-specific molecular motor. In neurons, KIF17 is responsible for the specialized transport of the N-methyl-D-aspartate receptor NR2B subunit, the glutamate receptor GluR5, and the potassium channel Kv4.2 from the cell body to the dendrites(PMID: 23762210).

实验方案

Product Specific Protocols
WB protocol for KIF17 antibody 85046-1-RRDownload protocol
IHC protocol for KIF17 antibody 85046-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols