验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IHC detected in | human colon cancer, human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19245-1-AP targets KIF18A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | KIF18A fusion protein Ag6166 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kinesin family member 18A |
| 别名 | PPP1R99, OK/SW-cl.108, MS-KIF18A, Kinesin-like protein KIF18A |
| 计算分子量 | 102 kDa |
| 观测分子量 | 102 kDa |
| GenBank蛋白编号 | BC048347 |
| 基因名称 | KIF18A |
| Gene ID (NCBI) | 81930 |
| RRID | AB_10598464 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NI77 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIF18A belongs to the Kinesin family. KIF18A binds to microtubules and moves along them using energy from ATP hydrolysis. KIF18A is related to the localization of membrane organelles and protein complexes, as well as the maintenance of microtubule dynamics and mitosis. KIF18A expression is low in normal tissues and high in solid tumors, including breast cancer, hepatocellular carcinoma and lung adenocarcinoma, colorectal cancer, renal cell carcinoma, and head and neck squamous cell carcinoma. The molecular weight of KIF18A is 102 kDa (PMID: 36830695).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIF18A antibody 19245-1-AP | Download protocol |
| IHC protocol for KIF18A antibody 19245-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget High KIF18A expression correlates with unfavorable prognosis in primary hepatocellular carcinoma. | ||
Front Genet Prognostic Biomarker KIF18A and Its Correlations With Immune Infiltrates and Mitosis in Glioma. | ||
Dis Markers Kinesin Family Member 18A (KIF18A) Contributes to the Proliferation, Migration, and Invasion of Lung Adenocarcinoma Cells In Vitro and In Vivo.
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Cell Div Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis.
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