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KIF19 Polyclonal antibody

KIF19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 19551-1-AP

Print datasheet

Synonyms

Kinesin-like protein KIF19

验证数据展示

  • WB analysis of human brain using 19551-1-AP

    WB analysis of human brain using 19551-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19551-1-AP (KIF19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19551-1-AP

    IHC staining of human brain using 19551-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19551-1-AP (KIF19 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 19551-1-AP

    IHC staining of human brain using 19551-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19551-1-AP (KIF19 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 19551-1-AP

    IF Staining of HepG2 using 19551-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIF19 antibody 19551-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19551-1-AP targets KIF19 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 19
Synonyms Kinesin-like protein KIF19
Calculated Molecular Weight 111 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberNM_153209
Gene Symbol KIF19
Gene ID (NCBI) 124602
RRIDAB_10640802
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2TAC6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF19 belongs to the kinesin-like protein family. The antibody recognizes isoform1 of KIF19 only.

实验方案

Product Specific Protocols
WB protocol for KIF19 antibody 19551-1-APDownload protocol
IHC protocol for KIF19 antibody 19551-1-APDownload protocol
IF protocol for KIF19 antibody 19551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Mol Cell Biol

Production of transgenic mice by random recombination of targeted genes in female germline stem cells.

Authors - Zhang Yong Y
View Article
human,mouseWB,IF,IP

J Biol Chem

AC6 regulates the microtubule-depolymerizing kinesin KIF19A to control ciliary length in mammals.

Authors - Kavisha Arora
View Article