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  • KD/KO Validated

KIF1B Polyclonal antibody

KIF1B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15263-1-AP

Print datasheet

Synonyms

CMT2, CMT2A, CMT2A1, HMSNII, KIAA0591, KIAA1448, KIF1B, kinesin family member 1B, Kinesin like protein KIF1B, KLP



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15263-1-AP targets KIF1B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen KIF1B fusion protein Ag7263 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 1B
Synonyms CMT2, CMT2A, CMT2A1, HMSNII, KIAA0591, KIAA1448, KIF1B, kinesin family member 1B, Kinesin like protein KIF1B, KLP
Calculated Molecular Weight 204 kDa
Observed Molecular Weight 199 kDa, 204 kDa
GenBank Accession NumberBC001415
Gene Symbol KIF1B
Gene ID (NCBI) 23095
RRIDAB_2131412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60333
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF1B antibody 15263-1-APDownload protocol
IHC protocol for KIF1B antibody 15263-1-APDownload protocol
IP protocol for KIF1B antibody 15263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIP

J Hazard Mater

Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.

Authors - Yiyuan Kang
mouseIF

Elife

Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense.

Authors - Kelsey Michelle Wells
mouseWB

Exp Neurol

Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI

Authors - Gang Liu
humanWB

Cell Rep

Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells.

Authors - Don-Marc Franchini
  • KD Validated
ratWB

Cell Death Dis

AAV-mediated inhibition of ULK1 promotes axonal regeneration in the central nervous system in vitro and in vivo.

Authors - Vinicius Toledo Ribas
human

J Cell Biol

Inhibition of endolysosome fusion increases exosome secretion

Authors - Ganesh Vilas Shelke