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验证数据展示

  • WB analysis using 67190-1-Ig (same clone as 67190-1-PBS)

    WB analysis using 67190-1-Ig (same clone as 67190-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67190-1-Ig (KIF20A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67190-1-Ig (same clone as 67190-1-PBS)

    IHC staining of human breast cancer using 67190-1-Ig (same clone as 67190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KIF20A antibody included
  • IHC staining of human breast cancer using 67190-1-Ig (same clone as 67190-1-PBS)

    IHC staining of human breast cancer using 67190-1-Ig (same clone as 67190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KIF20A antibody included
  • WB analysis of HeLa using 67190-1-Ig (same clone as 67190-1-PBS)

    WB analysis of HeLa using 67190-1-Ig (same clone as 67190-1-PBS)

    WB result of KIF20A antibody (67190-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF20A transfected HeLa cells. This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KIF20A Monoclonal antibody, PBS Only

KIF20A Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67190-1-PBS

产品说明书

CloneNo. 2A8E3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MKlp2, Mitotic kinesin-like protein 2, Kinesin-like protein KIF20A, GG10_2, FLJ21151

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy KIF20A Ready-To-Use IHC Kit. KIF20A primary antibody included.

产品信息

67190-1-PBS targets KIF20A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KIF20A fusion protein Ag8916 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 kinesin family member 20A
别名 MKlp2, Mitotic kinesin-like protein 2, Kinesin-like protein KIF20A, GG10_2, FLJ21151
计算分子量 890 aa, 100 kDa
观测分子量 100 kDa
GenBank蛋白编号BC012999
基因名称 KIF20A
Gene ID (NCBI) 10112
RRIDAB_2882485
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95235
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.